The process of claim 1, wherein the fermentation harvest comprises S. The process of claim 1, wherein the second diafiltered polysaccharide is passed through endotoxin removal resin.Ģ2. The process of claim 17, wherein the tangential flow filtration further comprises passage through a 50-100 kDa membrane.Ģ1. The process of claim 18, wherein the tangential flow filtration comprises deoxycholate/EDTA/Ca-salt buffer.Ģ0. ![]() The process of claim 17, further comprising passing first diafiltered polysaccharide through a tangential flow filtration.ġ9. The process of claim 1, wherein the fermentation harvest comprises Gram-positive bacteria.ġ8. The process of claim 13, wherein the tangential flow filtration further comprises passage through a 50-100 kDa membrane.ġ7. The process of claim 14, wherein the tangential flow filtration comprises deoxycholate/EDTA/Ca-salt buffer.ġ6. The process of claim 13, further comprising passing first diafiltered polysaccharide through a tangential flow filtration.ġ5. The process of claim 1, wherein the fermentation harvest comprises Gram-negative bacterial.ġ4. The process of claim 1, wherein the passing through multimodal chromatographic resin is performed in tangential flow filtration mode.ġ3. The process of claim 1, wherein the passing through multimodal chromatographic resin is performed in flow-through mode, tangential flow filtration mode, and/or filtration mode.ġ2. The process of claim 1, wherein the impurities comprise proteins, nucleic acids, cell wall components and/or peptidoglycan.ġ1. The process of claim 1, wherein treating with an enzyme is performed with multiple different enzymes sequentially, singularly, or in combination.ġ0. The process of claim 1, wherein the enzyme comprises one or more of Benzonase, Mutanolysin/lysozyme, β-D-N-acetyl glucosaminidase and/or Proteinase K.ĩ. The process of claim 6, wherein the acid comprises acetic acid.Ĩ. The process of claim 5, wherein the pH adjustment comprises the addition of an acid.ħ. ![]() The process of claim 1, wherein clarifying is followed by pH adjustment to about pH 3.5-5.0.Ħ. The process of claim 2, wherein the bacterial cell surface polysaccharide comprises capsular polysaccharide and/or exopolysaccharide.Ĥ. The process of claim 1, wherein the polysaccharide comprises bacterial cell surface polysaccharide.ģ. ![]() A process of purifying polysaccharide comprising: providing a fermentation harvest of Gram-positive and/or Gram-negative bacteria clarifying the fermentation harvest with deoxycholate concentrating the clarified polysaccharide by a first diafiltration treating the first diafiltered polysaccharide with an enzyme to digest impurities precipitating the enzyme with acetic acid concentrating the polysaccharide by a second diafiltration passing the second diafiltered polysaccharide through multimodal chromatographic resin and/or endotoxin removal resin and collecting the purified polysaccharide.Ģ.
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